Recombinant Human Vitamin D-binding protein (GC), Truncated

The process of expressing the recombinant human GC protein in the yeast requires the recombinant DNA gene formed by the integration of encoding gene for the 19-474aa of the human GC protein and N-terminal 6xHis-SUMOSTAR tag sequence, the expression vector that the recombinant DNA gene inserts into, the yeast that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-SUMOSTAR-tagged recombinant GC protein was obtained. This recombinant GC protein is characterized by high purity (>90%, SDS-PAGE). This GC protein ran along the gel to the band of approximately 52 kDa molecular weight.GC is a gene providing instruction of making a protein named Vitamin D-binding protein (also abbreviated as VDB or DBP) in human and belongs to ALB/AFP/VDB family. DBP is a multifunctional protein that is well-conserved in the evolution of vertebrates. This protein plays a crtical role in vitamin D? metabolites. In addtion to transporting vitamin D metabolites, DBP is proposed to be involved in the transport of fatty acids, in complement C5a-mediated chemotaxis, and in the activation of macrophages.